The Twitter card cannot handle/display a JS embed.
A JS embed only works when the viewer loads the page.
A Twitter card useds the <meta name="twitter:player tag to determine what to show in the “preview”/validator/twitter embed.
You need to fix <meta name="twitter:player" content="https://player.twitch.tv/?channel=hihellorachel&html5&muted=true&quality=mobile" /> not add Javascript to the page.
So you need to fix the meta tag to something like
<meta name="twitter:player" content="https://player.twitch.tv/?volume=0.34&muted&channel=hihellorachel&parent=cards-dev.twitter.com&parent=twitter.com&parent=cards-frame.twitter.com&parent=tweetdeck.twitter.com" />
Which I hinted at in my original reply.