Is there a way to pull all channels (all users that are streaming at a certain time) on Twitch?

[BarryCarlyon]

@raquelsouzacarvalho1

I need to collect data from all channels that are live at a certain time

Get Streams:

Reference

@raquelsouzacarvalho1

In addition, I would also like to obtain data from users who are in the chats of these transmissions.

Not without authorisation

Get Chatters:

Reference

@raquelsouzacarvalho1

I need to collect data from all channels that are live at a certain time. In addition, I would also like to obtain data from users who are in the chats of these transmissions. Is there any API for this?

All of this sounds like you may need research authorization from Twitch. Please refer to the developer TOS.