Twitch is a bit half and half about following the RFC.
Since it’s not really IRC. It’s TMI (Twitch Messaging Interface), which implements and IRC compatible interface.
The other thing to consider is that your bot might wanna be avoiding “Walls of Text” in chat anyway so keeping a shorter message length, will look better for a Twitch Chat overall.
Gotta account for tags and other data attached to the message but I’ve not exactly looked into it too much myself since MOST of the messaging my bots send doesn’t get anywhere near the limits